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Titlebook: Capillary Electrophoresis-Mass Spectrometry; Methods and Protocol Christian Neusüß,Kevin Jooß Book 2022 Springer Science+Business Media, LL

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书目名称Capillary Electrophoresis-Mass Spectrometry
副标题Methods and Protocol
编辑Christian Neusüß,Kevin Jooß
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Capillary Electrophoresis-Mass Spectrometry; Methods and Protocol Christian Neusüß,Kevin Jooß Book 2022 Springer Science+Business Media, LL
描述.This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols... ..Authoritative and cutting-edge, .Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols .aims to provide highly v
出版日期Book 2022
关键词CE-DBDI; Porous Tip Interface; proteomics; metabolites; lipids
版次1
doihttps://doi.org/10.1007/978-1-0716-2493-7
isbn_softcover978-1-0716-2495-1
isbn_ebook978-1-0716-2493-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
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,Capillary Electrophoresis–Mass Spectrometry (CE-MS) by Sheath–Flow Nanospray Interface and Its Use cterization of biologics in the biopharmaceutical industry. The direct coupling of CE with MS is an attractive approach, in that the high separation capability of CE and the ultrasensitive detection and accurate identification performance of MS can be combined to provide a powerful system for the an
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Successive Multiple Ionic-Polymer Layer Coatings for Intact Protein Analysis by Capillary Zone Elecften are applied to prevent adsorption and to determine the electroosmotic flow (EOF), which is of major importance for the separation in CE. Successive multiple ionic-polymer layer (SMIL) coatings are frequently used for protein analysis in capillary electrophoresis resulting in high separation eff
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Bottom-Up Analysis of Proteins by Peptide Mass Fingerprinting with tCITP-CZE-ESI-TOF MS After Trypttically digested, mostly by trypsin, and the resulting peptides are then separated prior to their transfer to a mass spectrometer. The following protocol portrays a bottom-up method, which was optimized for the application of CZE-ESI-TOF MS. Protein denaturation is achieved by addition of 2,2,2-trif
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Assessment of Macro- and Microheterogeneity of Monoclonal Antibodies Using Capillary Zone Electrophlonal antibodies (mAbs). mAbs are complex molecules comprising different glycoforms and many other posttranslational modifications. In addition to this inherent microheterogeneity, misassembling of antibodies can take place during production contributing to their macroheterogeneity. CZE-MS is a vers
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High-Sensitivity Glycoproteomic Analysis of Biological Samples by CZE-ESI-MS,icroheterogeneity of a glycoprotein in a bottom-up approach. This chapter describes in detail the sample preparation procedures using a purified biological sample, prostate-specific antigen, as a model protein, including proteolytic digestion (trypsin). In addition, insights are provided into the st
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