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Titlebook: Cancer Drug Resistance; Methods and Protocol Marta Baiocchi Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive lice

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Neutron Radiography at Lucas Heightsel of BC through the generation and ex vivo culture of patient-derived organotypic tumor spheroids (PDOTS) in a 3D microfluidic device. Moreover, the real-time screening of conventional chemotherapy agents on cultured PDOTS is also described.
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https://doi.org/10.1007/978-3-540-87866-7ab specialist can perform the pipeline analysis of RNA-seq described in this chapter using the Maser platform and the Tag-Count Comparison Graphical User Interface (TCC-GUI). These are free of charge for scientific use.
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A 3D Biomimetic System for Testing Anticancer Drug Sensitivity,ce (MDR) mechanisms. This 3D culture model resembles in vivo conditions and provides relevant and reproducible results. It is easy to set up and allows for facile manipulation for downstream analyses. All these remarkable features make this 3D culture model a promising tool in drug discovery and cancer cell biology research.
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Generation and Culture of Organotypic Breast Carcinoma Spheroids for the Study of Drug Response in el of BC through the generation and ex vivo culture of patient-derived organotypic tumor spheroids (PDOTS) in a 3D microfluidic device. Moreover, the real-time screening of conventional chemotherapy agents on cultured PDOTS is also described.
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https://doi.org/10.1007/978-3-540-87866-7ancer-initiation potential and enhanced chemoresistance, and present a distinctive nongenetic and cell-autonomous gene expression profile shared across different tumor types. The use of our H2BeGFP pulse-chase method opens the possibility to study live SCCC in any growing tissue either cancerous or normal.
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https://doi.org/10.1007/978-3-540-87866-7ight be beneficial. This protocol focuses on the description of a sample processing workflow that allowed for concurrent isolation of CTC and ctDNA from the same source sample. This single tube approach enables simultaneous analysis of multiple biomarkers to better monitor cancer drug resistance.
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