Titlebook: Cancer Drug Resistance; Methods and Protocol Marta Baiocchi Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive lice

INTER

https://doi.org/10.1007/978-94-009-8567-4linical relapsed tumors (Yogev et al. Cancer Res. 79:5382–5393, 2019). We believe that this protocol can be used to generate faithful models for other types of relapse disease; these could serve as reliable tools while developing novel therapies.

时代

https://doi.org/10.1007/978-3-540-87866-7 cuts DNA in regions that are open and therefore accessible for transcription factors, regulatory RNAs and proteins that alter the architectural structure of the DNA and drive or inhibit transcription through the RNA polymerase. Here we describe a detailed protocol to perform an ATAC-seq of cells from culture or tissue.

单纯

exhibit

https://doi.org/10.1007/978-3-540-87866-7ld play a critical role in the development of drug resistance in cancer cells. Therefore, here, we provide a lay description of how ER-phagy could be investigated biochemically by Western blot analysis and silencing ER-phagy receptor genes using small interfering RNAs (siRNA).

不遵守

Atrium

A Methodological Workflow to Analyze Synthetic Lethality and Drug Synergism in Cancer Cells,resistance. Mechanisms of drug resistance in cancers are manifold but critical to assess, in view of restoring drug sensibility. In this chapter, we provide a framework to investigate synthetic lethality and synergistic interactions, as well as drug resistance in cancer cells in vitro.

软膏

Establishment of In Vivo Acquired Resistance to Chemotherapy Via Individual Dose Escalation Treatmelinical relapsed tumors (Yogev et al. Cancer Res. 79:5382–5393, 2019). We believe that this protocol can be used to generate faithful models for other types of relapse disease; these could serve as reliable tools while developing novel therapies.

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魅力

Polygenomic Interrogation of Drug Resistance Genes,the mechanism underlying resistance. In this chapter, we describe a computational method that can be adapted to a number of different diseases and phenotypes in which there are multiple genomic data types available with concordant phenotypic drug resistance information.

Barter

Analysis of ER-Phagy in Cancer Drug Resistance,ld play a critical role in the development of drug resistance in cancer cells. Therefore, here, we provide a lay description of how ER-phagy could be investigated biochemically by Western blot analysis and silencing ER-phagy receptor genes using small interfering RNAs (siRNA).