Titlebook: Cancer Drug Resistance; Methods and Protocol Marta Baiocchi Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive lice

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Simultaneous Mapping of Enhancers and Enhancer Rearrangements with Paired-End H3K27ac ChIP-seq, map enhancers and their activity and to identify structural variations at enhancers. Since changes in enhancer activity and new enhancer translocations both play a major role in tumor initiation, progression, and response to therapy, this approach holds promise to uncover some of the mechanisms beh

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RNA Sequencing Data Analysis on the Maser Platform and the Tag-Count Comparison Graphical User Inteof RNA-seq output data are indispensable for research, but bioinformatics experts are not always available to assist. Currently, however, even a wet-lab specialist can perform the pipeline analysis of RNA-seq described in this chapter using the Maser platform and the Tag-Count Comparison Graphical U

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Polygenomic Interrogation of Drug Resistance Genes,otherapy. Pharmacogenomic methods seek to understand the interaction of genomic variation and response to chemotherapeutic treatment. This chapter presents a workflow to interrogate multiple genomic inputs and individually assess their relationship with the phenotype of drug resistance using hierarc

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Analysis of ER-Phagy in Cancer Drug Resistance,o develop resistance to chemotherapies. Autophagy and more specifically organelle specific autophagy is one such adaptive mechanism that promotes drug resistance in cancer cells. Endoplasmic reticulum–specific autophagy or ER-phagy has been more recently described to overcome ER-stress through the d

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https://doi.org/10.1007/978-94-009-7043-4facile visualization of cells with light microscopy and the ability to add (or subtract) drugs or biomolecules to interrogate the system or modulate the cellular response. Finally, the approach allows for terminal immunostaining of either (or both) cell types.

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