Titlebook: Cancer Cell Signaling; Methods and Protocol David M. Terrian Book 2003 Humana Press 2003

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Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD)it allows the measurement of, in parallel, the activities of three phospholipases utilizing aliquots derived from . biological sample. The approach for immunoprecipitation described in this chapter can be extrapolated to the study of a large array of enzyme isoforms. Finally, the intact-cell assays

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Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinoged by adding to EMSA an antibody specific for that protein, which will result in supershift complexes on the gel. The presence of the proteins in question can be further verified by standard Western analysis of the nuclear proteins.

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1064-3745 olution of this field is provided by an analysis of the Human Genome Project data, which reveal a previously unsuspected diversity in the multigene families encoding for most signaling pathway 978-1-61737-341-1978-1-59259-356-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/, Assay

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1064-3745 ly maintain a critical balance between cellular proliferation, differentiation, senescence, and death. One current research challenge is to identify those aberrations in signal transd- tion that directly contribute to a loss of this division-limited equilibrium and the progression to malignant trans

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Manfred Laucht,Daniel Brandeis,Katrin Zohseled paraffin-embedded tissue sections. This . detection system can be used to correlate apoptosis with specific antigen expression to identify factors involved in the induction and execution of the apoptotic pathway of cell death in a wide variety of experimental systems.

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