Titlebook: Cancer Cell Culture; Methods and Protocol Dania Movia,Adriele Prina-Mello Book 2023 The Editor(s) (if applicable) and The Author(s), under

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Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research, we describe a methodology to generate 3D liver spheres from pluripotent stem cells. Liver spheres are composed of three key liver cell types (hepatic progenitor cells, endothelial cells, and hepatic stellate cells) and have been used to study human cancer cell metastasis.

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Book 2023ensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional cancer cell culture, cell models at the Air-Liquid Interface, and the latest advancements in three-dimensional complex spheroid models and dedicated disease animal models. Part Two

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1064-3745 ation advice from the experts.This volume explores the latest collection of cell models that are used in preclinical cancer research, and covers both two-dimensional and three-dimensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional c

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