Titlebook: Cancer Cell Culture; Methods and Protocol Dania Movia,Adriele Prina-Mello Book 2023 The Editor(s) (if applicable) and The Author(s), under

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书目名称Cancer Cell Culture影响因子(影响力)




书目名称Cancer Cell Culture影响因子(影响力)学科排名




书目名称Cancer Cell Culture网络公开度




书目名称Cancer Cell Culture网络公开度学科排名




书目名称Cancer Cell Culture被引频次




书目名称Cancer Cell Culture被引频次学科排名




书目名称Cancer Cell Culture年度引用




书目名称Cancer Cell Culture年度引用学科排名




书目名称Cancer Cell Culture读者反馈




书目名称Cancer Cell Culture读者反馈学科排名

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Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Esophageal Adenocar by chronic irradiation of esophageal adenocarcinoma cells with clinically relevant doses of X-ray radiation. We also characterize cell cycle, apoptosis, reactive oxygen species (ROS) production, DNA damage and repair in this model to investigate the underlying molecular mechanisms of radioresistance in esophageal adenocarcinoma.

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Developing an In Vitro Isogenic Model of Chemotherapy-Resistant Lung Cancerenge for oncologists. In order to study and elucidate the cellular and molecular mechanisms implicated in the development of drug resistance in cancer, the use of isogenic models provides a valuable in vitro tool for investigating novel biomarkers and the identification of potential targetable pathways involved in drug-resistant cancers.

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Culturing Human Lung Adenocarcinoma Cells in a Serum-Free Environmentading to possible reproducibility issues in experiments and readouts. This chapter describes how to transition A549 cells to FBS-free medium and gives some insights on the further characterizations and functionality assays that would be necessary to perform for the validation of the cultured cells.

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A Method for Culturing 3D Tumoroids of Lung Adenocarcinoma Cells at the Air–Liquid Interfaceck polarity. Our method allows to overcome this limitation by enabling to grow tumoroids of lung adenocarcinoma cells and healthy lung fibroblasts at the Air–Liquid Interface (ALI). This ensures straightforward access to both the apical and basal surface of the cancer cell culture, with several advantages in drug screening applications.

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Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cfugation. The cells obtained using the protocol described can be further purified for a variety of cellular, immunological, molecular, and functional assays. In addition, these cells can be cryopreserved and bio-banked for future research studies.

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Joan P. Schwartz,Ann M. Marini,R. W. Rodieck, we describe a methodology to generate 3D liver spheres from pluripotent stem cells. Liver spheres are composed of three key liver cell types (hepatic progenitor cells, endothelial cells, and hepatic stellate cells) and have been used to study human cancer cell metastasis.