Titlebook: Calcium Signaling Protocols; David G. Lambert,Richard D. Rainbow Book 2013Latest edition Springer Science+Business Media, LLC 2013 Calcium

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Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2bed in this volume. Whole cell suspensions are loaded with Fura-2 and then placed into a cuvette-based fluorimetric system (measuring 510 nm emission at alternating 340/340 nm excitation). Cells can be stimulated with agonists and antagonists to enable temporal response profiling and concentration–r

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Confocal Microscopy: Theory and Applications for Cellular Signalingful approach to examine cellular structure and function. Allied with the development of suitable tools, it is now possible to interrogate a wide range of structural and functional aspects on both fixed and live cells. Here we describe the basic principles underlying confocal microscopy and provide m

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Ratiometric Ca2+ Measurements Using the FlexStation®Scanning Fluorometer dual-wavelength fluorescent probes. The FlexStation uses a Xenon flashlamp and monochromators for both excitation and emission light to allow the use of a wide range of fluorescent indicators. The system incorporates a fluid transfer system for addition of test compounds from a source plate to the

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Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®)onal plate readers is the ability to measure fluorescence emission from multiple wells (96 wells or 384 wells) simultaneously and with high temporal resolution. Consequently, FLIPR has been used extensively to record dynamic intracellular processes such as changes in intracellular Ca. ion concentrat

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Ratiometric [Ca2+]i Measurements in Adherent Cell-Lines Using the NOVOstar Microplate Readere of cellular events. Consequentially, experimental measurement of [Ca.]. is a potent technique for the medical science laboratory. The NOVOstar microplate reader is a versatile system, which may be easily configured to measure [Ca.].. Moreover, the relatively low cost of this system makes it an att

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Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterolle cells, heart pacemaker tissue and endocrine cells. Whole-cell recording is a functional electrophysiological assay that allows real-time measurement of macroscopic VSCC activity at the level of single cells. Using this technique, it is possible to probe the molecular physiology, pharmacology, and

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