Titlebook: CRISPR-Cas Methods; M. Tofazzal Islam,Pankaj K. Bhowmik,Kutubuddin A. Book 2020 Springer Science+Business Media, LLC, part of Springer Na

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Delivery of Cas9/sgRNA RNP into Wheat Microspores Using Synthetic CPP for Genome Editing and Gene Elowing for rapid and efficient generation of crop varieties with novel or improved traits. Despite wide applicability of the technology, a challenge in bringing edited crops to market is the lack of regulatory alignment between jurisdictions (e.g., EU). Additionally, technical difficulties in effici

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CRISPR-Cas9-Mediated Gene Editing in Wheat: A Step-by-Step Protocol,uable for understanding the function of genes but also offers an efficient approach for genetic manipulation and crop improvement. However, the efficiency of gene editing in wheat (. L.) is highly variable because of the genetic complexity of this plant species including 17 gigabases of genetic code

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Homology-Directed Transgene-Free Gene Editing in ,-based transformation techniques are prohibitively inefficient and lead to predominantly nonhomologous (i.e. off-target) integration. Standard CRISPR-based gene editing protocols have proved too ineffective to enable routine application. We have found that the use of CRISPR-Cpf1 in conjunction with

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Der Entwicklungsgang des Menschen,thin a single nucleotide difference to the wild-type sequence) to quickly ascertain the number of bases inserted or deleted on each allele of the targeted gene, which can then be followed by sequencing homozygous progeny of the initial regenerated transformants.

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1949-2448 .aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism..978-1-0716-0618-6978-1-0716-0616-2Series ISSN 1949-2448 Series E-ISSN 1949-2456