Titlebook: CRISPR-Cas Methods; M. Tofazzal Islam,Pankaj K. Bhowmik,Kutubuddin A. Book 2020 Springer Science+Business Media, LLC, part of Springer Na

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书目名称CRISPR-Cas Methods影响因子(影响力)




书目名称CRISPR-Cas Methods影响因子(影响力)学科排名




书目名称CRISPR-Cas Methods网络公开度




书目名称CRISPR-Cas Methods网络公开度学科排名




书目名称CRISPR-Cas Methods被引频次




书目名称CRISPR-Cas Methods被引频次学科排名




书目名称CRISPR-Cas Methods年度引用




书目名称CRISPR-Cas Methods年度引用学科排名




书目名称CRISPR-Cas Methods读者反馈




书目名称CRISPR-Cas Methods读者反馈学科排名

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Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific ExpressioNA double-strand breaks. However, up to now its application was mostly restricted to nonhomologous end-joining-mediated targeted mutagenesis. In contrast, precise genome modifications using a suitable donor sequence for homologous still pose a particular challenge in plants, as NHEJ is the dominant

aesthetic

Rice Gene Knockout or Downregulation through CRISPR-Cas9, a powerful tool for improving abiotic and biotic stress tolerance and increasing yield in rice. Valuable agronomical traits and functional study of a novel gene can be obtained by targeting single or multiple mutations in the rice genome. Here we describe the sgRNA design, cloning, and assembly of

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拱形大桥

CRISPR-Cas9-Mediated Genome Editing of the Model Grass Species ,explored in the model grass species . Selection of a single-guide RNA based on location in the gene and low risk of potential off-target effects is initially presented, with the goal of generating INDELS in a single-gene target. Next, the use of the polycistronic t-RNA/gRNA system to simultaneously

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An Optimized RNA-Guided Cas9 System for Efficient Simplex and Multiplex Genome Editing in Barley (,re created mainly by random mutagenesis, which produced many undesirable mutations and genomic rearrangements. With the emergence of programmable site-specific nucleases, mutations can be introduced precisely at specific genomic locations. CRISPR-Cas9 technology represents the most versatile and eff

语源学

octogenarian

Cloning-Free (DNA-Free) CRISPR-Cas9-Mediated Gene Editing in Human Liver Cell Line and Its Detectioditing. The recent efforts of scientists allow to improve current genome engineering techniques, which are used to generate modified cell lines for functional studies. Here we describe, in detail, the protocol for DNA-free CRISPR-Cas9-mediated gene editing in a human liver cell line. Briefly, we use