Titlebook: CRISPR Guide RNA Design; Methods and Protocol Tudor A. Fulga,David J. H. F. Knapp,Quentin R. V. Book 2021 Springer Science+Business Media,

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书目名称CRISPR Guide RNA Design影响因子(影响力)




书目名称CRISPR Guide RNA Design影响因子(影响力)学科排名




书目名称CRISPR Guide RNA Design网络公开度




书目名称CRISPR Guide RNA Design网络公开度学科排名




书目名称CRISPR Guide RNA Design被引频次




书目名称CRISPR Guide RNA Design被引频次学科排名




书目名称CRISPR Guide RNA Design年度引用




书目名称CRISPR Guide RNA Design年度引用学科排名




书目名称CRISPR Guide RNA Design读者反馈




书目名称CRISPR Guide RNA Design读者反馈学科排名

隐士

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978-1-0716-0689-6Springer Science+Business Media, LLC, part of Springer Nature 2021

Jingoism

Tudor A. Fulga,David J. H. F. Knapp,Quentin R. V. Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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https://doi.org/10.1057/9780230245099onsiderations for the design of short guide (sg) RNAs include the assessment of possible off-target activities, the prediction of on-target efficacies and mutational outcome. Manual design of sgRNAs taking into account these parameters, however, remains a difficult task. Thus, computational tools to

委托

https://doi.org/10.1057/9780230245099ine, and gene therapy. However, CRISPR nucleases can cleave off-target sites as well as on-target sites, causing unwanted mutations. Furthermore, after CRISPR treatments are delivered into cells or organisms, it is important to estimate the resulting mutation rates and to determine the patterns of m

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FRONT

The Thin Filament in Insect Flight Muscleing promise as a therapeutic for patients with genetic diseases. Multiple different CRISPR systems have been identified, each with its own target DNA recognition sequence, expanding the editable mammalian genome. It is also now appreciated that chemically modified nucleic acids can substitute for un

脱毛

The Thin Filament in Insect Flight Muscleby enzymatic reactions, however the process becomes time-consuming as the number of sgRNAs increases and does not allow the incorporation of chemical modifications that can improve or expand the functionality of CRISPR. Solid-phase RNA synthesis can overcome these issues, but highly pure full-length