Titlebook: CCN Proteins; Methods and Protocol Masaharu Takigawa Book 2017 Springer Science+Business Media New York 2017 CCN proteins.Cyr61 (Cysteine-r

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BUMP

Expression of CCN Genes and Proteins in Human Skin: Methods and Protocolsantitative information regarding relative levels of the proteins. Reliable determinations of the expression levels of CCN genes and proteins are essential to uncovering their roles in skin physiology and pathology.

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Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cellsal procedure using SYBR Green I for the quantitative analysis of CCN family gene expression. Especially, we describe the extraction and purification steps for RNA derived from mesenchymal cells, such as chondrocytes and osteoblasts that produce a large amount of extracellular matrix in detail.

火车车轮

Production of Recombinant CCN2 Protein in ,. Degradation and mis-folding are major problems in producing active recombinant CCN2. The method shown in this chapter describes the appropriate conditions under which we can produce CCN2 and its truncated fragments in ..

Anthropoid

In Vitro Transfection with and Expression of CCN Family of GenesIn this chapter, we describe an in vitro method for stable and inducible expression of CCN protein in a chondroprogenitor cell line and in chondrocytes in primary culture that does not involve the use of any viral vector.

桉树

PHIL

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https://doi.org/10.1007/3-540-28495-8is chapter, we provide our protocol for immunohistochemistry optimized for paraffin-embedded sections after having determined the optimal conditions for the use of antibodies against each member of the CCN family.

PTCA635

Anwendung in der Nachwuchsförderunginding partners and to find new binding partners whose affinities need to be determined. This chapter provides helpful tips to use the instrument for determination of the affinities of binding between CCN proteins and their binding partners.

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