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Titlebook: CCN Proteins; Methods and Protocol Masaharu Takigawa Book 2017 Springer Science+Business Media New York 2017 CCN proteins.Cyr61 (Cysteine-r

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书目名称CCN Proteins
副标题Methods and Protocol
编辑Masaharu Takigawa
视频videohttp://file.papertrans.cn/221/220234/220234.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: CCN Proteins; Methods and Protocol Masaharu Takigawa Book 2017 Springer Science+Business Media New York 2017 CCN proteins.Cyr61 (Cysteine-r
描述 This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques. Chapters details practical tips to overcome any obstacles with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences and pharmacology of CCN proteins. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and cutting-edge, .CCN Proteins: Methods and Protocols. .will be a valuable resource for a wide audience, ranging from the experienced CCN researchers looking for new approaches to junior graduate students just beginning in CCN research..
出版日期Book 2017
关键词CCN proteins; Cyr61 (Cysteine-rich 61); cysteine-rich secreted proteins; fibrosis; malignancy
版次1
doihttps://doi.org/10.1007/978-1-4939-6430-7
isbn_softcover978-1-4939-8197-7
isbn_ebook978-1-4939-6430-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
The information of publication is updating

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Analysis of Signaling Pathways Activated by CCN Proteinst cells. In this chapter, we describe our protocol examining the effects of these proteins on signal transduction pathways, especially mitogen-activated protein kinase cascades, activated by CCN member proteins, which examinations have been carried out mainly by using Western blotting methodologies.
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CCN Proteins978-1-4939-6430-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
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https://doi.org/10.1007/978-3-662-25293-2 family of signaling regulators, which may be referred to as “signal conductors.” Relevance to pathology is also briefly introduced. The information provided in this overview should be useful for readers of the following chapters.
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https://doi.org/10.1007/3-540-28495-8n a variety of cells under various conditions. In this chapter, we describe our Western blotting protocol to estimate protein production profiles of CCN family members after having assessed the specificity of the antibodies against each CCN member protein to ensure no cross-reaction with other CCN member proteins.
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Nachweisvermögen von Analysenverfahren Described herein are methods of constructing plasmids for CCN protein production (IGFBP-, VWC-, TSP-, and CT-domain) with . as a host, cultivation methods for protein production, and methods of purification for domain proteins using his-tag.
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https://doi.org/10.1007/978-3-8350-5541-4t cells. In this chapter, we describe our protocol examining the effects of these proteins on signal transduction pathways, especially mitogen-activated protein kinase cascades, activated by CCN member proteins, which examinations have been carried out mainly by using Western blotting methodologies.
发表于 2025-3-23 08:35:09 | 显示全部楼层
https://doi.org/10.1007/978-1-4939-6430-7CCN proteins; Cyr61 (Cysteine-rich 61); cysteine-rich secreted proteins; fibrosis; malignancy
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