Titlebook: C. elegans; Methods and Applicat Gal Haspel,Anne C. Hart Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exc

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Isolating , from the Natural Habitat, Here we provide a simple protocol on how to sample and rapidly identify . wild isolates. We outline how to find suitable habitats and organic substrates, followed by describing isolation and identification of . live cultures based on easily recognizable morphological characteristics, molecular barc

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Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in ,mals during imaging, quantifying behavior, and automating screens. However, challenges to their widespread adoption in the field include increased complexity over conventional methods, operational problems (such as clogging, leaks, and bubbles), difficulty in obtaining or fabricating devices, and th

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Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser,nique was pioneered in . where it led to exciting discoveries in the fields of development and neurobiology. All neurons studied so far in . can regenerate and regrow axons and dendrites after injury, allowing studies of the molecular and cellular basis of neuroregeneration. In this chapter, we desc

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Location Microformats: GEO and ADR introductory or advanced neuroscience undergraduate courses where students learn to understand the relationship between genetic mutations and behavior through using chemotaxis assays. In addition, instructions for students for the lab module are provided in Subheading “Example of Teaching Lab Module for Students”.

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Cryopreservation of , and Other Nematodes with Dimethyl Sulfoxide and Trehalose,t survive a typical freeze–thaw cycle. Here I describe an alternative method based on the popular combination of DMSO and trehalose as a cryoprotectant. This method allows the survival of large numbers of worms and effectively protects most developmental stages including adults.