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Titlebook: C. elegans; Methods and Applicat Gal Haspel,Anne C. Hart Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exc

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发表于 2025-3-21 16:12:30 | 显示全部楼层 |阅读模式
书目名称C. elegans
副标题Methods and Applicat
编辑Gal Haspel,Anne C. Hart
视频videohttp://file.papertrans.cn/221/220096/220096.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: C. elegans; Methods and Applicat Gal Haspel,Anne C. Hart Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exc
描述This detailed new edition provides a guide for implementing a selection of novel techniques utilizing the pioneering organism, .Caenorhabditis elegans., a vital discovery engine for the rules of life, from molecules and genes, through cellular physiology, metabolism and aging, neurobiology and behavior, to individuality and interaction with the environment. Beginning with two chapters offering practical advice for setting up research programs with C. elegans, either for a new research group or for a short-term project such as a teaching laboratory, the volume continues with numerous hands-on methodologies. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .C. elegans: Methods and Applications, Third Edition. offers the kind of clear guidance that will serve all researchers working with these vital and dynamic organisms..
出版日期Book 2022Latest edition
关键词Caenorhabditis elegans; Nematode; Optogenetics; Genetic tools; Research laboratories
版次3
doihttps://doi.org/10.1007/978-1-0716-2181-3
isbn_softcover978-1-0716-2183-7
isbn_ebook978-1-0716-2181-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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The Process of Developing Microformatsar expansion). The epitope-preserving ExCel protocol enables imaging of endogenous proteins with off-the-shelf antibodies, at a ~ 100 nm resolution (~2.8× linear expansion). The iterative ExCel protocol allows readout of fluorescent proteins at ~25 nm resolution (~20× linear expansion). The protocol
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1064-3745 nown pitfalls. .Authoritative and up-to-date, .C. elegans: Methods and Applications, Third Edition. offers the kind of clear guidance that will serve all researchers working with these vital and dynamic organisms..978-1-0716-2183-7978-1-0716-2181-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neutated variants with specificity for cyclic AMP. These can be used to regulate intracellular levels of cGMP and cAMP, which are important second messengers in sensory and other neurons. We further show how they can be combined with cyclic nucleotide-gated channels in two-component optogenetics, for d
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Book 2022Latest editionfalls. .Authoritative and up-to-date, .C. elegans: Methods and Applications, Third Edition. offers the kind of clear guidance that will serve all researchers working with these vital and dynamic organisms..
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