Titlebook: Bacterial Extracellular Vesicles; Methods and Protocol Steven Jay,Nicholas Pirolli Book 2024 The Editor(s) (if applicable) and The Author(s

驾驶

Book 2024icle (BEVs).  Chapters are divided into four parts focusing on characterization of Bacterial Extracellular Vesicles, assessment of Bacterial Extracellular Vesicle Biological Function In Vitro, preparation and functionalization of therapeutic Bacterial Extracellular Vesicles, and delivery and trackin

兽皮

不透明

Rat Fecal Metabolomics-Based Analysisological matrices ex vivo. We outline methods for growth, isolation, quantification, and labeling, as well as techniques for tracking bEV motion ex vivo and quantifying these data. The methods described here are relevant to bEV communication with host cells as well as drug delivery applications using bEVs.

退出可食用

https://doi.org/10.1007/978-1-4939-7643-0for improved BEV purification. We have previously found this method can reduce co-isolated protein impurities and potentiate anti-inflammatory bioactivity of probiotic BEVs. Thus, this method holds promise as a scalable alternative for improved BEV purification.

山崩

不可思议

Encumber

Analysis of Bacterial Extracellular Vesicles by Immunogold Transmission Electron Microscopyling with direct visualization of BEVs. Several challenges are commonly encountered during immunogold TEM analysis of BEVs, most notably inefficient antibody labeling and poor contrast. Here, we present an optimized protocol for immunogold TEM analysis of BEVs that overcomes such challenges.

GUEER

Multiple Particle Tracking: A Method for Probing Biologically Relevant Mobility of Bacterial Extraceological matrices ex vivo. We outline methods for growth, isolation, quantification, and labeling, as well as techniques for tracking bEV motion ex vivo and quantifying these data. The methods described here are relevant to bEV communication with host cells as well as drug delivery applications using bEVs.

先兆

Separation of Bacterial Extracellular Vesicles via High-Performance Anion Exchange Chromatographyfor improved BEV purification. We have previously found this method can reduce co-isolated protein impurities and potentiate anti-inflammatory bioactivity of probiotic BEVs. Thus, this method holds promise as a scalable alternative for improved BEV purification.

绝种

Spray Drying of Bacterial Membrane Vesicles for Vaccine Deliverytability without compromising their immunogenic protective effect. We discuss some of the most important experiments to characterize the generated spray-dried bacterial membrane vesicle powder vaccine.