Titlebook: Bacterial Extracellular Vesicles; Methods and Protocol Steven Jay,Nicholas Pirolli Book 2024 The Editor(s) (if applicable) and The Author(s

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Protein Status of ‘Small for Date’ Animalssecondary structure content in various media, including the membrane environment. In this chapter, we present how CD applications can be used to analyze the interaction of proteins with bacterial outer membrane vesicles (OMVs). Most CD studies characterizing the structure of proteins inserted into m

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Samantha Riccadonna,Pietro Franceschi bacteria. Cellular transport, communication, pathogenesis, and host-pathogen interactions are some of the major biological processes impacted by BEVs. Among these, host-pathogen interactions and bacterial pathogenesis are emerging as highly important targetable avenues underlined by the issues of a

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Hector C. Keun,Toby J. Athersuchions. Bacterial membrane vesicles are shed from both Gram-negative and Gram-positive bacteria and harbor many virulence factors, nuclear material, polysaccharides, proteins, and antigenic determinants, which are essential for immune recognition and evasion. Hence, bacterial membrane vesicles are ver

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George G. Harrigan,Royston Goodacrelular defense, pathogenesis, and signaling, among other functions. The functionality of OMVs can be enhanced by engineering developed for biomedical and biochemical applications. Here, we describe methods for directed packaging of enzymes into bacterial OMVs of . using engineered molecular systems,

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Seetharaman Vaidyanathan,Royston Goodacrese pathogens. However, antigen display on OMVs can be challenging to control and highly variable due to bottlenecks in protein expression and localization to the bacterial host cell’s outer membrane, especially for bulky and complex antigens. Here, we describe methods related to a universal vaccine

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https://doi.org/10.1007/978-1-0716-3247-5d microbe-host interactions. In tracking their biodistribution in vivo, BEVs can cross several physical host barriers including the intestinal epithelium, vascular endothelium, and blood-brain-barrier (BBB) to ultimately accumulate in tissues such as the liver, lungs, spleen, and the brain. This tis