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Titlebook: Bacterial Chromatin; Methods and Protocol Remus T. Dame Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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High-Resolution Characterization of DNA/Protein Complexes in Living Bacteriavage (ChEC), coupled with ligation-mediated polymerase chain reaction (LM-PCR) and Southern blot analysis. As an example, we apply these in vivo protein-mapping methods to . to show direct binding of architectural proteins in the Lac repressor-mediated DNA repression loop.
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High-Throughput Mapping of Chromosomal Conformations in , Under Physiological Conditions Using Massicontact. The procedure described here will suffice to provide a comprehensive map of transposition frequencies between tens of thousands of loci in a bacterial genome, with the resolution limited by the diversity of the insertion site library used and the sequencing depth applied.
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Visualization, Quantification, and Statistical Evaluation of Dynamics for DNA-Binding Proteins in Ban dynamics in mutant cells or cells exposed to changes in environmental conditions..In this chapter, we describe the preparation of . cells, the recording of movies of those cells expressing a monomeric variant of a yellow fluorescent protein (mNeonGreen) fused to a protein of choice, and the subseq
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