Titlebook: Bacterial Chromatin; Methods and Protocol Remus T. Dame Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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GeF-seq: A Simple Procedure for Base-Pair Resolution ChIP-seqir resolution. GeF-seq detects binding sites of DBPs as sharp peaks and thus makes it possible to identify the recognition sequence in each “binding peak” more easily and accurately compared to the common ChIP-seq.

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ChIP-qPCR of FLAG-Tagged Proteins in Bacteriaibe ChIP-qPCR. We provide a step-by-step protocol for the preparation of a ChIP library of a 3× FLAG-tagged protein in bacteria, describe how downstream qPCR experiments can be performed with the appropriate controls, and explain how the data is analyzed. This chapter provides reliable technical guidance for ChIP-qPCR studies in bacteria.

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