Titlebook: Brain Tumor Stem Cells; Methods and Protocol Sheila K. Singh,Chitra Venugopal Book 2019 Springer Science+Business Media, LLC, part of Sprin

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EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF,gnificant therapeutic benefits for therapeutic evaluation. For the identification of such an elusive and rare subpopulation of cells, a single cell analysis technology with deep profiling capabilities known as Mass Cytometry (CyTOF) can prove to be highly useful. CyTOF circumvents the spectral overl

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Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells,s9 system has now successfully been applied for genetic screens in many cell types, providing a powerful tool for functional genomics with manifold applications. Genome-wide guide-RNA (gRNA) libraries allow facile generation of a pool of cells, each harboring a gene knockout mutation that can be use

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Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci,ortant to express the mutated genes at appropriate levels, in relevant cell types, and in the proper developmental context. For recurrent mutations found in the heterozygous state in tumors, expression of the mutation from the endogenous locus is a more physiologically relevant recapitulation of the