Titlebook: Brachypodium Genomics; Methods and Protocol Gaurav Sablok,Hikmet Budak,Peter J. Ralph Book 2018 Springer Science+Business Media LLC 2018 im

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Genomic Approaches to Analyze Alternative Splicing, A Key Regulator of Transcriptome and Proteome Dfied conserved AS patterns among monocot and dicot plants. In this chapter, using a Brachypodium-virus pathosystem as a research template, we provide an overview of genomic and bioinformatic tools that can be used to investigate constitutive and alternative splicing in plants.

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A Highly Efficient and Reproducible , spp. Inoculation Method for ,,assay that facilitates quick screening of germplasm (e.g., mutant lines). Here, we provide an efficient and reproducible . infection assay for .. We believe this method will help further develop . as a model for genetic improvement of disease resistance in cereals against . pathogens.

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TILLING in ,,ethods including capillary electrophoresis. Here, we introduce the development of the mutant population BRACHYLIFE and present a Brachypodium TILLING protocol based on fluorescing primers for PCR, enzymatic cleavage, and detection with Applied Biosystems 3130xl Genetic Analyzer.

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Protocol for Coexpression Network Construction and Stress-Responsive Expression Analysis in ,,modules to map these key genes as e-QTLs. High throughput sequencing approaches has revolutionized the mining of the gene behavior and also the association of the genes over time-series. The present protocol chapter presents a unified workflow to understand the transcriptional modules in . using weighted coexpressed gene network analysis approach.

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Book 2018etabolomics, transcriptomics, genomics, and tilling. This book also explores techniques to study the widening genetic base of .Brachypodium. that will help researchers better understand the model plant using NGS technologies. Written in the highly successful .Methods in Molecular Biology .series for

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