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Titlebook: Bone Marrow Environment; Methods and Protocol Marion Espéli,Karl Balabanian Book 2021 Springer Science+Business Media, LLC, part of Springe

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Bone Marrow Environment978-1-0716-1425-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
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https://doi.org/10.1007/978-94-017-4851-3n differentiate in a variety of cell types such as osteoblasts, chondrocytes and adipocytes. The isolation of MSCs has been carried out by many studies that aim to control their differentiation into cartilaginous and bone cells in vitro in order to use this technology in the repair of damaged tissue
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https://doi.org/10.1007/978-94-017-4851-3lecular components required for their lifelong maintenance and differentiation. Although HSCs have been extensively analyzed and characterized, their ex vivo expansion, which constitutes a promising approach for therapeutic development in regenerative medicine, remains challenging. Here, we describe
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https://doi.org/10.1007/978-94-010-2770-0rvest a good quantity of BMSCs with good viability using fluorescence-activated cell sorting (FACS). Here, we describe the methods to effectively isolate BMSCs for flow cytometry analyses and subsequent FACS. Use of transgenic reporter lines facilitates FACS-based isolation of BMSCs, aiding to uncov
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