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Titlebook: Bone Marrow Environment; Methods and Protocol Marion Espéli,Karl Balabanian Book 2021 Springer Science+Business Media, LLC, part of Springe

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期刊全称Bone Marrow Environment
期刊简称Methods and Protocol
影响因子2023Marion Espéli,Karl Balabanian
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Bone Marrow Environment; Methods and Protocol Marion Espéli,Karl Balabanian Book 2021 Springer Science+Business Media, LLC, part of Springe
影响因子.This volume brings together classical and cutting-edge protocols on the spatio-temporal study of the cellular subsets constituting the bone and the marrow in both mouse and human. Chapters details methods on bone marrow (BM) ecosystem, to label, sort, analyse, and culture specific cell subsets as well as techniques allowing the evaluation of the function of some of the cellular elements of the BM. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... ..Authoritative and cutting-edge, .Bone Marrow Environment: Methods and Protocols .aims to help new investigators to pursue the characterization of the BM microenvironment in the coming years..
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Intravital Imaging of Bone Marrow Microenvironment in the Mouse Calvaria and Tibiae surgical approach, and introduce a bone shaving-based tibial imaging as a complementary method. To demonstrate the applicability of our method we used Lyz2-EGFP transgenic mice to track bone marrow neutrophil activities as an example.
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Literature and the Authority of Technology,genitor cells remains essential in the analysis of OCL biology, underlining the importance of reliable gold standard protocols to be applied throughout OCL research. This chapter will deal with in vitro differentiation of OCLs from murine bone marrow cells, as well as isolated monocytes and dendriti
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Analysing Literature through Filmshree dimensions at high resolution. Here, we provide a detailed experimental protocol for the generation of thick slices of BM from murine femoral cavities, the immunostaining of cellular and structural components within these samples, and their optical clearing, which enhances the depth at which op
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1064-3745 d cutting-edge, .Bone Marrow Environment: Methods and Protocols .aims to help new investigators to pursue the characterization of the BM microenvironment in the coming years..978-1-0716-1427-3978-1-0716-1425-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Willie van Peer,Aikaterini Nousipermitted in-depth studies of HSC niches in mice. Here, we describe our protocol for visualizing and analyzing the localization, morphology, and function of niche components in the mouse calvarium, using combined confocal and two-photon intravital microscopy, and we present the specific example of measuring vascular permeability.
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