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Titlebook: Bioreactors in Stem Cell Biology; Methods and Protocol Kursad Turksen Book 2016 Springer Science+Business Media New York 2016 Biomaterials.

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期刊全称Bioreactors in Stem Cell Biology
期刊简称Methods and Protocol
影响因子2023Kursad Turksen
视频video
发行地址Includes cutting-edge methods and protocols for bioreactors in stem cell research.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
学科分类Methods in Molecular Biology
图书封面Titlebook: Bioreactors in Stem Cell Biology; Methods and Protocol Kursad Turksen Book 2016 Springer Science+Business Media New York 2016 Biomaterials.
影响因子This meticulous volume recognizes the need to translate what has been learned primarily in tissue culture dishes to approaches supporting scale-up studies, not only to large quantities of cells but also to heterogeneous cell constructs.  Notable advances are being made in these latter approaches, prompting this collection of a variety of representative protocols that facilitate important modifications and novel approaches to bioreactors in stem cell research, contributed by both established and new investigators in this area. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Practical and authoritative, .Bioreactors in Stem Cell Biology: Methods and Protocols. will serve as an ideal guide for scientists seeking to increase our understanding of stem cells and their potential to repair and regenerate tissues and organs..
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Gabriel Flynn,David Smith,Mary Kirwanase of up to 4.2 over a period of 5 days compared to a maximum 1.5–2-fold increase in the adherent static culture over a 1 week period. In the bioreactor culture, these cells maintained self-renewal, karyotype stability, and cloning efficiency capabilities. This approach can be also used for human n
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Multicompartmental Hollow-Fiber-Based Bioreactors for Dynamic Three-Dimensional Perfusion Culture,livers oxygen and carbon dioxide for pH regulation. Such bioreactors are available at various dimensions ranging from 0.2 to 800 mL cell compartment volumes (manufactured by StemCell Systems, Berlin, Germany). Here, we describe in detail the setup and maintenance of a small-scale 4-chamber bioreacto
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Uniform Embryoid Body Production and Enhanced Mesendoderm Differentiation with Murine Embryonic Steissue development and organogenesis. This chapter describes a protocol for using a rotary suspension bioreactor to produce embryoid bodies and process the differentiation of mouse embryonic stem cells (mESCs), and to assess the efficiency of EB differentiation in the bioreactor by real-time PCR and
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Use of Stirred Suspension Bioreactors for Male Germ Cell Enrichment,andling. Here, we describe the use of stirred suspension bioreactors, as a novel system for enriching undifferentiated germ cells from 1-week-old pigs. This method capitalizes on the adherent properties of somatic cells within a controlled environment, thus promoting the enrichment of progenitor cel
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