Titlebook: Biophysical approaches to translational control of gene expression; Jonathan D. Dinman Book 2013 Springer Science+Business Media New York

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978-1-4899-8709-9Springer Science+Business Media New York 2013

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X-Ray Analysis of Prokaryotic and Eukaryotic Ribosomes,s of the bacterial ribosome in different functional states (Frank et al. 1995; Stark et al. 1997a, b; Agrawal et al. 1998). However, not until the X-ray crystallographic structures of the entire 70S ribosome as well as the individual 30S and 50S subunits began to emerge did accurate atomic models be

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Methods for Studying the Interactions of Translation Factors with the Ribosome,oteins but not about their ribosomal position will not be discussed, while X-ray crystallography and Cryo-EM are described in . and ., respectively. Special emphasis is given to directed hydroxyl radical probing, since this approach can provide not only information about the overall binding site of

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,Analyses of RNA–Ligand Interactions by Fluorescence Anisotropy,ion mechanisms and discuss statistical validation methods. Finally, we have included sections on the use of plate readers for measuring fluorescence anisotropy across large numbers of samples and their application to high-throughput drug screening. Fluorescence anisotropy has been used to elucidate

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A Multidisciplinary Approach to RNA Localisation,ification of the binding preferences of .-acting factors by cross-linking and immunoprecipitation and NMR-based approaches as well as the computational prediction of RNA secondary and tertiary structure combined with the use of super-resolution microscopy methods. Moreover, molecular modelling and c

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Biophysical approaches to translational control of gene expression

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Late Cold War Literature and Cultures of the bacterial ribosome in different functional states (Frank et al. 1995; Stark et al. 1997a, b; Agrawal et al. 1998). However, not until the X-ray crystallographic structures of the entire 70S ribosome as well as the individual 30S and 50S subunits began to emerge did accurate atomic models be

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