Titlebook: Biomimetics and Stem Cells; Methods and Protocol Gordana Vunjak-Novakovic,Kursad Turksen Book 2014 Springer Science+Business Media New York

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Determining the Role of Matrix Compliance in the Differentiation of Mammary Stem Cells,protocol includes detailed methods for the fabrication of substrata with varying stiffness, culture of mammary progenitor cells on synthetic substrata, pharmacological modulation of actomyosin contractility, and analysis of gene expression to define the resulting fate of human mammary stem cells.

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Cultivation of Human Bone-Like Tissue from Pluripotent Stem Cell-Derived Osteogenic Progenitors in tent stem cells (collectively termed PSCs) into mesenchymal-like progenitors, and their subsequent cultivation on decellularized bovine bone scaffolds in perfusion bioreactors, to support the development of viable, stable bone-like tissue in defined geometries.

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https://doi.org/10.1007/978-3-030-93203-9of early specialized vascular cells from human pluripotent stem cells, to differentiate these toward mature endothelial cells and pericytes, and to utilize a collagen scaffold to facilitate organization into vascular networks.