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Titlebook: Biomimetics and Stem Cells; Methods and Protocol Gordana Vunjak-Novakovic,Kursad Turksen Book 2014 Springer Science+Business Media New York

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期刊全称Biomimetics and Stem Cells
期刊简称Methods and Protocol
影响因子2023Gordana Vunjak-Novakovic,Kursad Turksen
视频video
发行地址Includes cutting-edge methods and protocols on the use of biomimetic systems with stem cells.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advic
学科分类Methods in Molecular Biology
图书封面Titlebook: Biomimetics and Stem Cells; Methods and Protocol Gordana Vunjak-Novakovic,Kursad Turksen Book 2014 Springer Science+Business Media New York
影响因子.Biomimetics and Stem Cells: Methods and Protocols. collects a series of approaches to demonstrate the role and value of biomimetics for the better understanding of stem cell behavior and the acceleration of their application in regenerative medicine. Recent advances in tissue engineering are enabling scientists to ‘‘instruct’’ stem cells toward differentiating into the right phenotypes, in the right place and at the right time. Given these advances, biomimetic environments are being designed to recapitulate, in vitro, the combinations of factors known to guide tissue development and regeneration in vivo and thereby help unlock the full potential of the stem cells. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Practical and essential, .Biomimetics and Stem Cells: Methods and Protocols. focuses on the use of biomimetic systems for stem cells in order to aid in moving this vital field of study forward.
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High-Throughput Cell Aggregate Culture for Stem Cell Chondrogenesis,re enables screening of various soluble factors for their effects on stem cell function and chondrogenesis. In this protocol, we report a platform that allows the formation of stem cell aggregates in a 96-well plate format. Specifically, stem cells are centrifuged to form high-density pellets, mimic
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Multiwell Plate Tools for Controlling Cellular Alignment with Grooved Topography, alignment of cytoskeletal components, in fact, precedes whole cell alignment. A variety of methods exist to manipulate cytoskeletal and whole cell alignment; one of the simplest and most predictable involves seeding adherent cells onto defined substrate topography. We present here two methods to cr
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Determining the Role of Matrix Compliance in the Differentiation of Mammary Stem Cells,sociated with pregnancy and lactation. The ability of mammary stem cells to maintain themselves as well as to give rise to differentiated progeny is not only affected by soluble factors but has increasingly become linked to mechanical cues including the elastic modulus of the extracellular matrix (E
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Biomimetic Strategies Incorporating Enzymes into CaP Coatings Mimicking the In Vivo Environment, growth. In this chapter we present a successful method to coating biodegradable natural-origin materials with biomimetic CaP layer as a strategy to incorporate enzymes with the main aim of controlling and tailoring their degradation over time. This strategy seems to enhance the bone-bonding, osteoc
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