Titlebook: Biomedical Engineering Technologies; Volume 2 Avraham Rasooly,Houston Baker,Miguel R. Ossandon Book 2022 The Editor(s) (if applicable) and

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https://doi.org/10.1057/9780230106284ngle molecule sensitivity, can probe how membrane receptors are organized under both normal and pathological conditions. While a number of receptors have been extensively studied in cultured cells, technical challenges have largely impeded their robust quantification in tissue samples. To rigorously

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Xiaochun Li,Ping He,Yu Zhou,Zheyu Dongthat are toxic and carcinogenic, such as chloroform and methyl-tert-butyl ether for lipidomics, or caustic chaotropic solutions for genomics and transcriptomics, such as guanidine or urea. A common preservation solution for pathology is formaldehyde, which is a carcinogen. Use of acetonitrile as a u

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Xiaochun Li,Yuanting Xu,Dianshuang Wangamage and damage to the sugar-phosphate backbone of DNA. Double Strand Breaks (DSBs) are the most severe form of DNA damage and if left unchecked, may precipitate genomic rearrangements, cell death or contribute to malignancy. In clinical contexts, radiation is often used to induce DSBs as a form of

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Method to Isolate Dormant Cancer Cells from Heterogeneous Populationsgy 05:1–10, 2017; Reátegui et al. J Mater Chem B 2:7440–7448, 2014). Isolated DCCs can then be recovered from the gel and analyzed. Here we describe this immobilization method that can be used to isolate DCCs from heterogeneous cell populations that may also include dormancy-incapable cancer cells a

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Label-Free Morphological Phenotyping of In Vitro 3D Microtumorsing MATLAB program. With this method, we have been able to show structural and spatial heterogeneity among single cells and surrounding regions in tumor spheroids, and between different cell types in tumor-fibroblast co-cultured spheroids. Our method has the potential to both bridge the gap between

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Microdissection Methods Utilizing Single-Cell Subtype Analysis and the Impact on Precision MedicineSynergy between microdissection and droplet digital PCR (ddPCR) enhances the molecular analysis. We demonstrate here a methodology that illustrates genotyping of a solid tumor from a small tissue biopsy sample in a time- and cost-efficient manner, using immunostain targeting as an example.

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