Titlebook: Bioluminescent Imaging; Methods and Protocol Christian E. Badr Book 2014 Springer Science+Business Media New York 2014 Aequorin.Bioluminesc

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Split , Luciferase for Imaging Ligand–Receptor Bindingn cell-based assays and living mice. While we illustrate this imaging method for chemokine ligand CXCL12 and its receptors CXCR4 and CXCR7, this imaging strategy can be generalized to a large number of ligand–receptor interactions.

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Real-Time Bioluminescent Tracking of Cellular Population Dynamicsmated, non-destructive tracking of cellular population dynamics in real-time. This method, which relies on the detection of a continuous bioluminescent signal produced through expression of the bacterial luciferase gene cassette, provides a low cost, low time-intensive means for generating additional data compared to alternative methods.

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https://doi.org/10.1007/978-1-62703-718-1Aequorin; Bioluminescence imaging; Bioluminescent reporters; Firefly luciferase; Gaussia luciferase; Imag

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Christian E. BadrIncludes cutting-edge methods and protocols to examine bioluminescent imaging.Provides step-by-step, easy to follow detail essential for reproduction.Contains key notes and implementation advice from

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/187781.jpg

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https://doi.org/10.1007/978-3-642-97938-5Mycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the . luciferase reporter.