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Titlebook: Bioluminescent Imaging; Methods and Protocol Christian E. Badr Book 2014 Springer Science+Business Media New York 2014 Aequorin.Bioluminesc

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发表于 2025-3-21 19:58:08 | 显示全部楼层 |阅读模式
期刊全称Bioluminescent Imaging
期刊简称Methods and Protocol
影响因子2023Christian E. Badr
视频video
发行地址Includes cutting-edge methods and protocols to examine bioluminescent imaging.Provides step-by-step, easy to follow detail essential for reproduction.Contains key notes and implementation advice from
学科分类Methods in Molecular Biology
图书封面Titlebook: Bioluminescent Imaging; Methods and Protocol Christian E. Badr Book 2014 Springer Science+Business Media New York 2014 Aequorin.Bioluminesc
影响因子.Bioluminescent Imaging: Methods and Protocols. distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Bioluminescent Imaging: Methods and Protocols. aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with s
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Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporterechniques. Imaging of Ca. indicators is widely used to record transient intracellular Ca. increases associated with bioelectrical activity. The natural bioluminescent Ca. sensor aequorin has been historically the first Ca. indicator used to address biological questions. Aequorin imaging offers sever
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Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cellase (GLase) as a reporter protein. The luminescence signals produced by the enzymatic reaction of secreted GLase (luciferase) and coelenterazine (luciferin) are detected with an electron-multiplying charge-coupled device camera. An exocytotic event of protein secretion can be visualized using the pr
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Bioluminescence Reporter Gene-Based Detection of MicroRNAsse small RNAs have been demonstrated to play crucial roles in a variety of biological processes including cell differentiation, proliferation, and survival. Knowledge of specific expression patterns of miRNAs is critical for functional studies. Here, we describe a bioluminescence reporter gene-based
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The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent , Bearing Transgenicimeric protein consisting of HIF-1α oxygen-dependent degradation domain (ODD) fused to luciferase was ubiquitously expressed in all tissues. Hypoxic stress leads to the accumulation of ODD-luciferase in the tissues of this mouse model which can be identified by noninvasive bioluminescence measuremen
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