Titlebook: Bioluminescence; Methods and Protocol Sung-Bae Kim Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive

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https://doi.org/10.1007/978-3-8350-5403-5grown in the presence of the molecules of interest in a 96-well microplate, and 24 h later, BL is determined with a simple protocol lacking washing steps, using cost-efficient reagents with a reasonable readout time for high-throughput applications.

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https://doi.org/10.1007/978-3-8350-5403-5tion and then culturing the filter in an agar medium, the beer-derived ATP remaining on the filter can be removed and the ATP of LAB cells is increased. As a result, the signal-to-noise ratio of the RMD method can be dramatically improved.

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A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules Against the Intracellular For microplate and a 24 h treatment. The assay setup shows excellent balance between simplicity (cell culture manipulation/infection and timing) and quality parameters, as well as potential to detect drug-like molecules acting in a fast and cytotoxic manner.

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