Titlebook: Bioluminescence; Methods and Protocol Sung-Bae Kim Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive

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A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening Against Infective African Trphic and infective (bloodstream) form of .. The assay relies on a bioluminescent transgenic parasite harboring a genetically encoded copy of a thermostable redshifted firefly luciferase from ...The major advantages of the assay are simplicity and cost efficiency, along with excellent quality parame

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Design of an Intron-Retained Bioluminescence Reporter and its Application in Imaging of Pre-mRNA Spl with abnormal splicing of pre-mRNA. Conventional methods require sample lysis and thus cannot be used for monitoring pre-mRNA splicing in real time. This chapter guides how to develop an intron-retained bioluminescence (BL) reporter, which simulates the splicing process of pre-mRNA in vitro and in

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Generation of Bi-Reporter-Expressing Tri-Segmented Arenavirusirus life cycle and also for the discovery of antiviral countermeasures. The plasmid-encoded viral ribonucleoprotein components initiate the transcription and replication of a plasmid-driven full-length viral genome, resulting in infectious virus. Thereby, this approach is ideal for the generation o

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https://doi.org/10.1007/978-3-658-09972-5cal phenomena both in vitro and in vivo. Coelenterazine (CTZ), the known widespread luciferin found in bioluminescent organisms, develops bioluminescence imaging (BLI). Here, we describe an approach to synthesize a series of novel CTZ derivatives for diversifying the toolbox of the BL substrates. Fu