Titlebook: Bioluminescence; Methods and Protocol Sung Bae Kim Book 2016Latest edition Springer Science+Business Media New York 2016 luciferase.optical

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Synthetic Bioluminescent Coelenterazine Derivativesssible applications. Here, we describe the synthesis of three C-6 substituted CTZ derivatives retaining the recognition by . luciferase (RLuc) and its derivatives. The novel derivatives are useful as bright blue-shifted CTZ derivatives, which can be used as an alternative to hitherto reported compound DeepBlueC™.

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Quantum Yield Determination Based on Photon Number Measurement, Protocols for Firefly Bioluminescencacterize luminescence light intensity emitted diffusively from the reaction solution mixture. Here, methods to measure number of photons to determine . according to the techniques of national radiometry standards are described. As an example, experiments using firefly bioluminescence reactions are introduced.

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Book 2016Latest editionreagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and practical, .Bioluminescence: Methods and Protocols, Third Edition. is a usefulcomplement to the first and second edition for new and experienced researchersalike.. .

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Marianne Inéz Lien,Jørgen Lorentzenroximation of the adjacent fragments of split-cpLuc recovering the enzyme activity. This probe design greatly improves signal-to-noise (S/N) ratios upon tracing weak protein–protein interactions (PPIs) in mammalian cells.

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Bioluminescent Ligand–Receptor Binding Assays for Protein or Peptide Hormones protein or peptide hormones for ligand–receptor interaction studies in future. In the present article, we provided detailed protocols for setting up the novel bioluminescent ligand–receptor binding assays using two representative protein hormones as examples.

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