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Titlebook: Bioluminescence; Methods and Protocol Sung Bae Kim Book 2016Latest edition Springer Science+Business Media New York 2016 luciferase.optical

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https://doi.org/10.1007/978-3-030-86320-3or GPCR signaling in a high-throughput manner. However, information related to the temporal reaction of GPCR with specific ligands remains poor. We recently developed a bioluminescence method for the quantitative detection of the interaction between GPCR and β-arrestin using split luciferase complem
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Bioluminescence978-1-4939-3813-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
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https://doi.org/10.1007/978-3-531-91140-3ssible applications. Here, we describe the synthesis of three C-6 substituted CTZ derivatives retaining the recognition by . luciferase (RLuc) and its derivatives. The novel derivatives are useful as bright blue-shifted CTZ derivatives, which can be used as an alternative to hitherto reported compound DeepBlueC™.
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Elias’ theoretisch-empirischer Rahmenacterize luminescence light intensity emitted diffusively from the reaction solution mixture. Here, methods to measure number of photons to determine . according to the techniques of national radiometry standards are described. As an example, experiments using firefly bioluminescence reactions are introduced.
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Sung Bae KimIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
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