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Titlebook: Biological Small Angle Scattering: Techniques, Strategies and Tips; Barnali Chaudhuri,Inés G. Muñoz,Volker S. Urban Book 2017 Springer Nat

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https://doi.org/10.1007/978-3-642-87189-4ough a three-step process of planning, preparing and performing a basic SAXS measurement. The minimal requirements necessary to prepare samples are described specifically for protein and other macromolecular samples in solution. We address the very important aspects in terms of sample characterizati
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https://doi.org/10.1007/978-3-642-49918-0ing between the sample and background can be achieved using dialysis methods but in biological SAXS of monodisperse systems, sample preparation is routinely being performed with size exclusion chromatography (SEC). SEC is the most reliable method for SAXS sample preparation as the method not only pu
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https://doi.org/10.1007/978-3-662-36560-1 of a multi-component biological assembly, as well as the spatial arrangements between the components. The large difference in the neutron scattering properties between hydrogen and deuterium is key to the method. Isotopic substitution of deuterium for some or all of the hydrogen in either the molec
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https://doi.org/10.1007/978-3-662-36560-1ing inhomogeneities at low resolution. An important example of such systems are solutions of biological macromolecules. Rapid development in the methodology for solution scattering data interpretation and model building during the last two decades brought the analysis far beyond the determination of
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Immunologische Herzerkrankungen,in cell regulation and signalling, and their malfunction is linked to severe pathologies. Understanding the functional role of disorder requires the structural characterization of IDPs and the complexes they form. Small-angle Scattering of X-rays (SAXS) and Neutrons (SANS) have notably contributed t
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https://doi.org/10.1007/978-3-642-16481-1e (SAXS) data. It is possible to accurately predict WAXS scattering on the basis of atomic coordinate sets and thus use it as a means of testing molecular models constructed on the basis of crystallography, molecular dynamics (MD), cryo-electron microscopy or . modeling. WAXS data may provide insigh
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Angeborene Herzfehler im Erwachsenenalter,w, the aggregation process starts from an ensemble of native states that convert into transiently formed oligomers, higher order assemblies and protofibrils and, finally, fibrils. The different species exist in equilibrium in solution leading to a high degree of sample heterogeneity. It is impossibl
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,Akute Herzinsuffizienz und Lungenödem,heir chemistry, biological macromolecules undergo structural changes over distances ranging from atomic to micrometer scales. X-ray and neutron scattering provide three key assets for tackling this challenge. First, they may be conducted on solutions where the macromolecules are free to sample the c
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