Titlebook: Biological Dosimetry; Cytometric Approache Wolfgang G. Eisert,Mortimer L. Mendelsohn Conference proceedings 1984 Springer-Verlag Berlin Hei

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Conference proceedings 1984rial within a biological system. Thus, for mutagens, one can speak of DNA dosimetry, meaning the number of adducts produced in the DNA of target cells such as bone-mar­ row stem cells or spermatogonia.

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arget material within a biological system. Thus, for mutagens, one can speak of DNA dosimetry, meaning the number of adducts produced in the DNA of target cells such as bone-mar­ row stem cells or spermatogonia.978-3-540-12790-1978-3-642-69334-2

Expertise

Flow Cytometric Measurement of Cellular DNA Content Dispersion Induced by Mutagenic Treatmentan increased variation of the cellular DNA content in the whole cell population. This effect can be observed in the flow-cytometric DNA distributions as broadening of the G./G.- and G.+M-phase peaks and can be quantitated. Work done along these lines will be briefly reviewed in the following.

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Flow Cytometric Analysis of Chromosome Damage After Irradiation: Relation to Chromosome Aberrations ograms and the analysis of these histograms by a computer program. Using this method we have determined changes in the distributions of chromosome frequency versus DNA content as a function of X-ray dose. The results are compared with dose effect relations for chromosome structural changes assessed

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Preliminary Reanalysis of Radiation-Induced Chromosome Aberrations in Relation to Past and Newly Revermore, we noted a difference in aberration frequencies between Hiroshima and Nagasaki: the frequency of aberrant cells was consistently higher in every dose range in Hiroshima than in Nagasaki. One possible explanation for this difference was thought to be the contribution of the neutron component

小丑

Cytometric Analysis of Mammalian Sperm for Induced Morphologic and DNA Content Errorsforts to develop quantitative morphologic analysis procedures. These procedures lend themselves to automation, so that once their value is established the time required for large numbers of analyses may be greatly reduced.

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