Titlebook: Bioconjugation; Methods and Protocol Sam Massa,Nick Devoogdt Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 P

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RaumFragen: Stadt – Region – Landschaftite-specific method for coupling fluorine-18-labeled carboxylate substrates with biomolecules. Methods for preparation of two [.F]fluorocarboxylates and their ligation to a specific peptide sequence (LAP peptide) are presented herein.

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Ergebnisse: Wirkungen im Umfeld,ible with existing conjugation chemistries. Here we describe a method to express recombinant proteins in . site-specifically incorporating 5-hydroxytryptophan, followed by the chemoselective labeling of this residue using a chemoselective rapid azo-coupling reaction.

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CRISPR/Cas9-Mediated Genetic Engineering of Hybridomas for Creation of Antibodies that Allow for Sie LPXTG is then acted on by the bacterial transpeptidase Sortase to site-specifically add on any of a number of chemical moieties that possess a triglycine repeat. This technique thus allows rapid production of an antibody onto which a wide array of chemical moieties can be site-specifically conjugated.

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Enzymatically Catalyzed Radiofluorination of Biomolecules,ite-specific method for coupling fluorine-18-labeled carboxylate substrates with biomolecules. Methods for preparation of two [.F]fluorocarboxylates and their ligation to a specific peptide sequence (LAP peptide) are presented herein.