Titlebook: Bioconjugation; Methods and Protocol Sam Massa,Nick Devoogdt Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 P

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Ergebnisse: Wirkungen im Umfeld, a powerful way to site-specifically introduce nonnatural chemical functionalities into recombinant proteins, which can be subsequently functionalized in a chemoselective manner. Even though several strategies currently exist to selectively label recombinant proteins in this manner, there is conside

细胞膜

,Problem – ein weiterer wichtiger Begriff,molecules for cell killing, or half-life extension modules for optimized pharmacokinetics. Although many conjugation strategies are straightforward to apply, most of them do not enable site-specific and orthogonal conjugation, and do not yield a defined stoichiometry. Moreover, techniques offering t

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Intelligenzforschung und Intelligenzmodelle,h, diagnostic and therapeutic applications. Many of these applications require the antibody to be labeled with additional chemical or biological moieties. Here, we describe a method for the rapid and site-specific labeling of nearly any “off-the-shelf” IgG. Our method utilizes small photoreactive an

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https://doi.org/10.1007/978-3-662-68927-1d architecture via conjugation. Telechelic proteins bearing the SpyTag and SpyCatcher reactive sequences can be expressed and purified for bioconjugation in vitro, giving protein conjugates, branched proteins, and circular proteins. By encoding both reactive sequences in the same construct for expre

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1064-3745 oubleshooting and avoiding known pitfalls. .Authoritative and practical, .Bioconjugation: Methods and Protocols. is an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery..978-1-4939-9656-8978-1-4939-9654-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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,Neurosen und psychosomatische Störungen,acterial trans-peptidase Sortase A (Srt A). Srt A efficiently mediates sequence-specific peptide ligation under mild conditions and has few undesirable side reactions. We first describe the production, purification and characterization of Srt A enzyme and a scFv construct which targets activated pla

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