Titlebook: Basophils and Mast Cells; Methods and Protocol Bernhard F. Gibbs,Franco H. Falcone Book 2020Latest edition Springer Science+Business Media,

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John Impagliazzo,Timo Järvi,Petri Pajutomated hematology analyzers and fluorescence flow cytometry. Manual basophil counting of peripheral blood smears is currently regarded as the reference method, although the limitations of this method (distribution, observer, and statistical errors) are widely recognized. Automated hematology analyz

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https://doi.org/10.1007/978-3-319-17145-6ning the allergens to be tested. These can be complex extracts, purified or recombinant allergens, or peptides. Older methods, such as the IgE CAP test, only allow sIgE measurements to multiple allergens in individual measurements. Newer technologies such as the ImmunoCAP. ISAC test allows semiquant

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https://doi.org/10.1007/978-3-319-17145-6e of a range of de novo synthesized and preformed mediators of the allergic response. β-Hexosaminidase release is usually measured as a simple readout for degranulation. Furthermore, the rat basophilic leukemia (RBL)-2H3 cell line is commonly used for measuring degranulation, monitoring β-hexosamini

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Mast Cell and Basophil Cell Lines: A CompendiumFcεRI), and to test cell-stabilizing compounds. In this chapter, we review the most widely used and better-characterized MC and basophil cell lines, highlighting their advantages and drawbacks. It must be pointed out, however, that while cell lines represent a useful in vitro tool due to their easy