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Titlebook: Basic and Advanced Laboratory Techniques in Histopathology and Cytology; Pranab Dey Textbook 2022Latest edition The Editor(s) (if applicab

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https://doi.org/10.1007/b101876rate amyloidosis amyloid material in the histopathology sections. Alkaline Congo red stain is the most commonly used stain of amyloid. Thioflavin T is a very sensitive technique. However, it is not a specific stain for amyloid.
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https://doi.org/10.1007/b101876d commonly by direct smear, centrifugation, cytocentrifugation, liquid-based preparation, millipore technique and cell block. This chapter presents a detailed description of these various techniques of processing the sample.
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Embedding of Tissue in Histopathologyaraffin at a constant temperature and a cold plate to cool the tissue block. Finally, the orientation of the tissue in the embedding medium is discussed as this is a vital part of getting helpful information from the tissue.
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Decalcification of Bony and Hard Tissue for Histopathology Processingssful decalcification of tissue depends on the assessment of the consistency of the tissue, the volume of the tissue, proper washing and fixation of tissue. In addition, the correct selection of the decalcifying agent and exact determination of the endpoint of the decalcification process is also essential.
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