Titlebook: Basic and Advanced Laboratory Techniques in Histopathology and Cytology; Pranab Dey Textbook 2022Latest edition The Editor(s) (if applicab

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affluent

Processing of Tissue in the Histopathology Laboratoryis to remove water from the tissue section and to impregnate the tissue with another medium that can give support to the tissue. The various factors influence tissue processing, such as the size of the tissue, temperature, viscosity, and negative pressure. Tissues are processed mainly by automated t

fabricate

带来的感觉

Decalcification of Bony and Hard Tissue for Histopathology Processingove calcium salt from the tissue without affecting the morphology of the tissue and staining. Removal of calcium salt can be done with the help of acid, chelating agents, ion-exchange resin and electrolysis method. The relative advantages and disadvantages of various decalcification methods have als

dendrites

Tissue Microtomy: Principle and Procedurethe tissue, and among those, the rotary microtome is most commonly used in the laboratory. This is a heavy and stable microtome, and good quality 2 to 3-μm thin section is possible. The chapter discusses the various types of knives and blades used in microtomy. Nowadays, disposable blades of low and

CANDY

Frozen Section: Principle and Proceduree cryostat is the instrument to freeze the tissue and also cut the frozen tissue into the microscopic section. The rapid freezing of the tissue sample converts the water into ice. The firm ice within the tissue acts as embedding media to cut the tissue. The frozen section is mainly used for rapid di

评论性

Staining Principle and General Procedure of Staining the Tissuefferent components of the tissue for interpretation. A chromogenic dye absorbs the light of a particular wavelength of the white light representing a specific colour and emits the light containing the rest of the colour. Therefore, we see a coloured light from the dye. The dye can be classified on t

intangibility

Haematoxylin and Eosin Stain of the Tissue Sectionovides good contrast. Haematoxylin also stains collagenous material, minerals, and myelin fibres. Hematoxylin is oxidised to haematin which is a weak anionic dye. When a metallic salt (mordant) is combined with haematin, then a cationic dye-metal complex is formed that behaves as a strong basic dye

条约

Cryptic

Connective Tissue Stain: Principle and Proceduret of the connective tissue includes collagen, reticulin, elastin and basement membrane. There are predominantly five types of collagen fibres, out of which collagen I carries the main bulk of connective tissue. Reticulin fibres are collagen III fibres, and they support the parenchymal tissue of the