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Titlebook: Basic Exercises in Immunochemistry; A Laboratory Manual Alois Nowotny Book 1979Latest edition Springer-Verlag Berlin Heidelberg 1979 Assay.

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,Shakespeare and the ‘Long Arras’,The products of H-2 genes are among the most potent transplantation antigens in mice. Much attention has been devoted to the isolation and chemical characterization of these antigens, particularly because of their importance in immunogenetic studies.
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Chromatographic Separation of the IgG FragmentsCarboxymethyl cellulose has been shown to be one of the most useful ion exchangers in protein chromatography, The procedure elaborated by Peterson and Sober (1956) as well as by Sober et al. (1956) gives good resolution of different proteins. This method has been applied by Porter (1959) for the separation of breakdown products of IgG globulin,
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Isolation of Cell Membranes From Ascites TumorsVarious methods are described in the literature for the isolation of membranes of nucleated cells. The procedure described in this exercise is based on the method of Boone et al. (1973) with minor modifications as applied to the isolation of TA3-Ha murine ascites tumor membrane in the author’s laboratory (Nowotny et al., 1976).
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Isolation of Murine Transplantation Antigen PreparationThe products of H-2 genes are among the most potent transplantation antigens in mice. Much attention has been devoted to the isolation and chemical characterization of these antigens, particularly because of their importance in immunogenetic studies.
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Honour, debt, the rejection and St Paul procedure for gamma globulin isolation, this is still the simplest and one of the most efficient methods if one does not wish to separate the different Immunoglobulin fractions from each other. This method gives a preparation which contains almost all of the Immunoglobulins together with other noni
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