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Titlebook: Basic Exercises in Immunochemistry; A Laboratory Manual Alois Nowotny Book 1979Latest edition Springer-Verlag Berlin Heidelberg 1979 Assay.

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楼主: clannish
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From , to ,: Jamesian Metamorphosis,atment of the enzyme does not interfere with its enzymatic activity and a high percentage of the oxidized enzyme can be bound to antibodies without interfering with the reactivity of the antibody. This procedure is given here, based on the description of Nakane and Kawaoi (1974).
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Enzymatic Labeling of Proteins with 125I the procedure used by Dr. Ah-kau Ng (NIH, National Cancer Institute, Bethesda, MD) to iodinate exposed proteins on human tumor cell surfaces, based on the publication of Phillips and Morrison (1971).
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Preparation of Peroxidase-Labeled Antibodyatment of the enzyme does not interfere with its enzymatic activity and a high percentage of the oxidized enzyme can be bound to antibodies without interfering with the reactivity of the antibody. This procedure is given here, based on the description of Nakane and Kawaoi (1974).
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Isolation of Immunoglobulins by Gel Filtration on Sephadex G 200 Columnfree in the liquid that fills the spaces between the swollen gel granules (void volume). For a detailed description of the gel filtration processes the students should consult specified literature (Flodin, 1962).
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