Titlebook: Basic DNA and RNA Protocols; Adrian J. Harwood Book 1996 Humana Press 1996

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Capillary Blotting of Agarose Gelsheir size in an agarose gel (.). The DNA is then partially cleaved by depurination (to facilitate the transfer of larger DNA fragments) and alkali denatured by sequential soaking of the gel in solutions containing HCl and NaOH, respectively. The denatured DNA fragments are then transferred to a soli

Pastry

Random Primed 32P-Labeling of DNA and Vogelstein (.), is a method of incorporating radioactive nucleotides along the length of a fragment of DNA. Random primed labeling can give specific activities of between 2 × 10. and 5 × 10. dpm/µg (..). The method below is essentially that described by Feinberg and Vogelstein (.) in which a DN

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Hybridization and Competition Hybridization of Southern Blots) have been described in the preceding chapters (.. and .). This chapter describes the detection of specific DNA sequences by hybridization to a labeled probe of complementary sequence. This method is suitable for the detection of a wide range of DNA concentrations down to single-copy genes within m

Cantankerous

Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizationshese methods offer an attractive alternative to “radioactively tagged” probes in terms of safety, cost, and efficiency. Most previous nonradioactive strategies utilized the detection of the modified base by the use of a coupled antibody- or avidin-alkaline phosphatase with subsequent exposure to Nit