Titlebook: Basic DNA and RNA Protocols; Adrian J. Harwood Book 1996 Humana Press 1996

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Kapil Bhalla,Celalettin Ustun,Warren Fiskusf nucleic acid at the expense of the other. Frequently, when cellular material is limiting, it is desirable to isolate both RNA and DNA from the same source. Such is the case for biopsy specimens, primary cell lines, or manipulated embryonic stem cells.

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刺耳

里程碑

https://doi.org/10.1007/978-3-642-82734-1in RNA quantity following developmental or physiological changes. Its disadvantage is that it cannot be used when the RNA sample contains additional homologous sequences, which will hybridize to the probe and confuse the results.

NAVEN

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Diastole

Native Polyacrylamide Gel Electrophoresisthis range, fragments are both difficult to separate and hard to visualize because of diffusion within the gel matrix. These problems are solved by native polyacrylamide gel electrophoresis (PAGE). Using native PAGE, fragments as small as 10 bp and up to 1 kb can be separated with a resolution of as little as 1 in 500 bp.

Oligarchy

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RNA Slot Blottingin RNA quantity following developmental or physiological changes. Its disadvantage is that it cannot be used when the RNA sample contains additional homologous sequences, which will hybridize to the probe and confuse the results.

言外之意

3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detectioner sensitivity applications, and the combination of fluorescein-labeling and enhanced chemiluminescent detection allows as little as 20 × 10. mol of homologous target to be detected within a 1-h exposure.