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Titlebook: Basic Confocal Microscopy; W. Gray (Jay) Jerome,Robert L. Price Book 2018Latest edition Springer Nature Switzerland AG 2018 fluorescence.m

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Introduction and Historical Perspective,icroscopy Workshop at several venues, we have found that students and technicians who are novice users of confocal microscopes are often instructed by their mentors to go to the confocal system and collect some images. Often the implied message is that it should be easy and quick since it is only a
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The Theory of Fluorescence,confocal microscope is used in conjunction with fluorescent samples. Fluorescence imaging provides a specific, high-contrast signal that maximally exploits the ability of the confocal microscope to remove out-of-focus light. For this reason, it is imperative for confocal microscopists to have a basi
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Specimen Preparation,hips along the optical (.) axis as well as in the .-. plane. In other words, the investigation of spatial relationships is being evaluated in at least three dimensions. Often, this involves the collection of a series of planar images along the .axis and the reconstruction of the data into an image t
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Confocal Digital Image Capture, this goal is to match, as closely as possible, the parameters of all hardware and software in the image collection train. For reasons outlined in this chapter, it is particularly important to match the resolution of your digital image to the output resolution from the microscope. In Chap. ., we dis
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Types of Confocal Instruments: Basic Principles and Advantages and Disadvantages,oken down into four basic categories: single-photon point-scanning confocal systems, multiphoton (nonlinear) point-scanning confocal systems, spinning disk confocal systems, and super-resolution systems. New developments are continually being added to the hardware and software of these microscopes t
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