Titlebook: Basic Confocal Microscopy; W. Gray (Jay) Jerome,Robert L. Price Book 2018Latest edition Springer Nature Switzerland AG 2018 fluorescence.m

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Types of Confocal Instruments: Basic Principles and Advantages and Disadvantages, disk confocal systems, and super-resolution systems. New developments are continually being added to the hardware and software of these microscopes to improve their performance, but the majority of confocal systems will fall into one of these groups.

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Analysis of Image Similarity and Relationship, determining image similarity including our perception of color in images. In addition, the correct use of several commonly accepted methods such as Pearson’s correlation coefficient, Manders’ overlap coefficient, and Spearman’s ranked correlation coefficient is discussed.

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Faalangst/examenvrees van (t)huis uit,ses, these basic principles are also applicable to wide-field fluorescence microscopy. The chapter also reviews factors that alter or limit the fluorescent signal generated by the sample and discusses new fluorescent probes being developed to overcome some issues related to low signal yield.

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Help for People Coping with Physical Change,eir data. This chapter provides 12 guiding principles that need to be addressed when collecting and processing digital images with confocal and other microscopes and a list of resources available for assistance with confocal imaging.

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The Theory of Fluorescence,ses, these basic principles are also applicable to wide-field fluorescence microscopy. The chapter also reviews factors that alter or limit the fluorescent signal generated by the sample and discusses new fluorescent probes being developed to overcome some issues related to low signal yield.

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