Titlebook: Basic Cloning Procedures; Valdis Berzins Book 1998 Springer-Verlag Berlin Heidelberg 1998 DNA.RNA.gene.nucleic acid.protein.protein synthe

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RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase,The purpose of this chapter is to introduce beginners in molecular biology to RNA transcription by phage T7 DNA-dependent RNA polymerase. The work outlined here includes the transcription procedure of plasmid vectors or PCR-amplified DNA templates, the purification and identification of RNA products by sequencing with reverse transcriptase.

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PCR-Based Site-Specific Mutagenesis,ant DNA technology. Nucleotide changes are necessary not only for the analysis of the structural basis of gene and corresponding protein function, but also for the generation of novel gene products. The availability of the polymerase chain reaction (PCR) in the last decade has enabled the modificati

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Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids,derable ease and speed. In an electric field nucleic acids move through the pores in the gel towards the anode according to their molecular weight. After electrophoresis, the location of nucleic acids within the gel can be directly visualized in ultraviolet light by a dye ethidium bromide. If desire

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Immunological Methods for Analysis of Recombinant Proteins,inant DNA in . cells many clones are usually produced, and the same situation appears if recombinant DNA expression libraries are available. Furthermore, if appropriate monoclonal or polyclonal antibodies are available, the method of immunoscreening of colonies for direct immunological detection of

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