期刊全称 | Basic Cloning Procedures | 影响因子2023 | Valdis Berzins | 视频video | | 发行地址 | Basic laboratory techniques, approved in several international molecular biology courses.Step-by-step protocols with detailed practical hints, troubleshooting guide and short background information.Th | 学科分类 | Springer Lab Manuals | 图书封面 |  | 影响因子 | This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints. | Pindex | Book 1998 |
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