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Titlebook: Basic Cell Culture Protocols; Jeffrey W. Pollard,John M. Walker Book 19972nd edition Humana Press 1997

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Ideenkampf und »pacifìke Mission«which represent 60–65% of the cells of the liver and about 80% of the volume of the organ (since they are larger than the other cells) (.–.). Two years after Howard et. al. (.) discovered that collagenase was a very important tool in the separation of liver cells, Berry and Friend (.) introduced the technique of collagenase perfusion of the liver.
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Rückblicke auf Personen und Zuständeolid culture system. These progenitor cells, initially called “Colony Forming Units in Culture” (CFU-C) developed into colonies after several days of culture in the presence of different types of conditioned media containing “hematopoietic growth factors.” They are found in bone marrow, blood, fetal liver, and spleen of most mammalian species.
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Ex Vivo Maintenance of Differentiated Mammalian Cells,d dynamically interacting sets of mechanisms: Mechanisms associated with epithelial-mesenchymal interactions, a relationship that is universal and that constitutes the organizational basis for all metazoan tissues; and mechanisms associated with stem cell biology and with stem cell-fed maturational lineages.
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Collagen Gel Culture of the Human Hematopoietic Progenitors CFU-GM, CFU-E, and BFU-E,olid culture system. These progenitor cells, initially called “Colony Forming Units in Culture” (CFU-C) developed into colonies after several days of culture in the presence of different types of conditioned media containing “hematopoietic growth factors.” They are found in bone marrow, blood, fetal liver, and spleen of most mammalian species.
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